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fmol ® dna cycle sequencing system kit  (Promega)

 
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    Promega fmol ® dna cycle sequencing system kit
    Fmol ® Dna Cycle Sequencing System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fmol+sequencing+kit/pmc01190211-138-38-45?v=Promega
    Average 90 stars, based on 1 article reviews
    fmol ® dna cycle sequencing system kit - by Bioz Stars, 2026-08
    90/100 stars

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    Defining the GlnR-binding sequence upstream of actII-ORF4. A, DNase I footprinting analysis of the sense strand of the actII-ORF4 promoter region. The upper two electropherograms present the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein. The lower electropherogram shows the <t>sequencing</t> map of the actII-ORF4 promoter region. The sequence protected from DNase I digestion is indicated. The supersensitive site is indicated by an asterisk (*). B, illustration of the sequence upstream of actII-ORF4. The sequence protected by GlnR is shaded, and the putative GlnR-binding site is indicated by bold and italic letters. The actII-ORF4 TSS is shown by a bent arrow. The putative −35 and −10 regions of the actII-ORF4 promoter are underlined. The translational start codon is indicated by a box. C, base mutations introduced into the GlnR-protected <t>DNA</t> sequence in the upstream region of actII-ORF4. D, EMSA analysis between GlnR and the mutated probes. In each reaction, 10 ng of the tested Cy5-labeled mutated probe was added. The wild-type actII-ORF4 probe (actII-ORF4p) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by an arrow or an open brace.
    Fmol Dna Cycle Sequencing Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Defining the GlnR-binding sequence upstream of actII-ORF4. A, DNase I footprinting analysis of the sense strand of the actII-ORF4 promoter region. The upper two electropherograms present the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein. The lower electropherogram shows the <t>sequencing</t> map of the actII-ORF4 promoter region. The sequence protected from DNase I digestion is indicated. The supersensitive site is indicated by an asterisk (*). B, illustration of the sequence upstream of actII-ORF4. The sequence protected by GlnR is shaded, and the putative GlnR-binding site is indicated by bold and italic letters. The actII-ORF4 TSS is shown by a bent arrow. The putative −35 and −10 regions of the actII-ORF4 promoter are underlined. The translational start codon is indicated by a box. C, base mutations introduced into the GlnR-protected <t>DNA</t> sequence in the upstream region of actII-ORF4. D, EMSA analysis between GlnR and the mutated probes. In each reaction, 10 ng of the tested Cy5-labeled mutated probe was added. The wild-type actII-ORF4 probe (actII-ORF4p) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by an arrow or an open brace.
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    Defining the GlnR-binding sequence upstream of actII-ORF4. A, DNase I footprinting analysis of the sense strand of the actII-ORF4 promoter region. The upper two electropherograms present the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein. The lower electropherogram shows the <t>sequencing</t> map of the actII-ORF4 promoter region. The sequence protected from DNase I digestion is indicated. The supersensitive site is indicated by an asterisk (*). B, illustration of the sequence upstream of actII-ORF4. The sequence protected by GlnR is shaded, and the putative GlnR-binding site is indicated by bold and italic letters. The actII-ORF4 TSS is shown by a bent arrow. The putative −35 and −10 regions of the actII-ORF4 promoter are underlined. The translational start codon is indicated by a box. C, base mutations introduced into the GlnR-protected <t>DNA</t> sequence in the upstream region of actII-ORF4. D, EMSA analysis between GlnR and the mutated probes. In each reaction, 10 ng of the tested Cy5-labeled mutated probe was added. The wild-type actII-ORF4 probe (actII-ORF4p) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by an arrow or an open brace.
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    fmol® dna cycle sequencing system kit  (Promega)
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    Promega fmol® dna cycle sequencing system kit
    Defining the GlnR-binding sequence upstream of actII-ORF4. A, DNase I footprinting analysis of the sense strand of the actII-ORF4 promoter region. The upper two electropherograms present the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein. The lower electropherogram shows the <t>sequencing</t> map of the actII-ORF4 promoter region. The sequence protected from DNase I digestion is indicated. The supersensitive site is indicated by an asterisk (*). B, illustration of the sequence upstream of actII-ORF4. The sequence protected by GlnR is shaded, and the putative GlnR-binding site is indicated by bold and italic letters. The actII-ORF4 TSS is shown by a bent arrow. The putative −35 and −10 regions of the actII-ORF4 promoter are underlined. The translational start codon is indicated by a box. C, base mutations introduced into the GlnR-protected <t>DNA</t> sequence in the upstream region of actII-ORF4. D, EMSA analysis between GlnR and the mutated probes. In each reaction, 10 ng of the tested Cy5-labeled mutated probe was added. The wild-type actII-ORF4 probe (actII-ORF4p) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by an arrow or an open brace.
    Fmol® Dna Cycle Sequencing System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    fmol® dna cycle sequencing system kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    Defining the GlnR-binding sequence upstream of actII-ORF4. A, DNase I footprinting analysis of the sense strand of the actII-ORF4 promoter region. The upper two electropherograms present the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein. The lower electropherogram shows the sequencing map of the actII-ORF4 promoter region. The sequence protected from DNase I digestion is indicated. The supersensitive site is indicated by an asterisk (*). B, illustration of the sequence upstream of actII-ORF4. The sequence protected by GlnR is shaded, and the putative GlnR-binding site is indicated by bold and italic letters. The actII-ORF4 TSS is shown by a bent arrow. The putative −35 and −10 regions of the actII-ORF4 promoter are underlined. The translational start codon is indicated by a box. C, base mutations introduced into the GlnR-protected DNA sequence in the upstream region of actII-ORF4. D, EMSA analysis between GlnR and the mutated probes. In each reaction, 10 ng of the tested Cy5-labeled mutated probe was added. The wild-type actII-ORF4 probe (actII-ORF4p) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by an arrow or an open brace.

    Journal: The Journal of Biological Chemistry

    Article Title: Direct Involvement of the Master Nitrogen Metabolism Regulator GlnR in Antibiotic Biosynthesis in Streptomyces *

    doi: 10.1074/jbc.M116.762476

    Figure Lengend Snippet: Defining the GlnR-binding sequence upstream of actII-ORF4. A, DNase I footprinting analysis of the sense strand of the actII-ORF4 promoter region. The upper two electropherograms present the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein. The lower electropherogram shows the sequencing map of the actII-ORF4 promoter region. The sequence protected from DNase I digestion is indicated. The supersensitive site is indicated by an asterisk (*). B, illustration of the sequence upstream of actII-ORF4. The sequence protected by GlnR is shaded, and the putative GlnR-binding site is indicated by bold and italic letters. The actII-ORF4 TSS is shown by a bent arrow. The putative −35 and −10 regions of the actII-ORF4 promoter are underlined. The translational start codon is indicated by a box. C, base mutations introduced into the GlnR-protected DNA sequence in the upstream region of actII-ORF4. D, EMSA analysis between GlnR and the mutated probes. In each reaction, 10 ng of the tested Cy5-labeled mutated probe was added. The wild-type actII-ORF4 probe (actII-ORF4p) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by an arrow or an open brace.

    Article Snippet: FAM-labeled M13F primer was also used for preparing DNA ladders with a fmol DNA cycle sequencing kit (Promega, Madison, WI).

    Techniques: Binding Assay, Sequencing, Footprinting, Recombinant, Labeling, Positive Control, Purification

    Defining the precise GlnR-binding motif upstream of redZ. A, DNase I footprinting analysis of the sense strand of the redZ promoter region. In the panel, the upper two electropherograms indicate the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein (GlnR). The lower electropherogram corresponds to the sequencing map of the redZ promoter region. The DNA sequence protected from DNase I digestion is presented. The putative GlnR-binding motif is indicated by red boxes. B, the sequence of the redZ promoter region. The conserved GlnR-binding motif is shaded. The redZ TSS is shown by a bent arrow. The putative −35 and −10 regions of the redZ promoter are underlined. The redZ translational start codon is boxed. C, illustrations of base mutations introduced into the GlnR-binding sequence upstream of redZ. D, EMSA analysis of binding of GlnR to the mutated probes. In each EMSA reaction, 10 ng of the Cy5-labeled mutated probe was added. The redZ wild-type probe (redZp) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by arrows.

    Journal: The Journal of Biological Chemistry

    Article Title: Direct Involvement of the Master Nitrogen Metabolism Regulator GlnR in Antibiotic Biosynthesis in Streptomyces *

    doi: 10.1074/jbc.M116.762476

    Figure Lengend Snippet: Defining the precise GlnR-binding motif upstream of redZ. A, DNase I footprinting analysis of the sense strand of the redZ promoter region. In the panel, the upper two electropherograms indicate the footprinting reactions with no protein or the addition of 1 μg of recombinant His6-GlnR protein (GlnR). The lower electropherogram corresponds to the sequencing map of the redZ promoter region. The DNA sequence protected from DNase I digestion is presented. The putative GlnR-binding motif is indicated by red boxes. B, the sequence of the redZ promoter region. The conserved GlnR-binding motif is shaded. The redZ TSS is shown by a bent arrow. The putative −35 and −10 regions of the redZ promoter are underlined. The redZ translational start codon is boxed. C, illustrations of base mutations introduced into the GlnR-binding sequence upstream of redZ. D, EMSA analysis of binding of GlnR to the mutated probes. In each EMSA reaction, 10 ng of the Cy5-labeled mutated probe was added. The redZ wild-type probe (redZp) was used as a positive control. The amounts of purified His6-GlnR (GlnR, μm) used are as indicated. Free DNA probes and GlnR-DNA complexes are shown by arrows.

    Article Snippet: FAM-labeled M13F primer was also used for preparing DNA ladders with a fmol DNA cycle sequencing kit (Promega, Madison, WI).

    Techniques: Binding Assay, Footprinting, Recombinant, Sequencing, Labeling, Positive Control, Purification